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anti glucose regulated protein 94  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology anti glucose regulated protein 94
    Anti Glucose Regulated Protein 94, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 255 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/glucose+regulated+protein+grp+94/GRP+94+Antibody/pmc11752926-52-64-69
    Average 94 stars, based on 255 article reviews
    anti glucose regulated protein 94 - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Membrane:

    Article Title: CXC195 induces apoptosis and endoplastic reticulum stress in human hepatocellular carcinoma cells by inhibiting the PI3K/Akt/mTOR signaling pathway.
    Article Snippet: Protein samples from the HepG2 cells extracts were separated using 8% SDS-PAGE (high molecular weight) or 10% SDS-PAGE (regular molecular weight) (Sigma-Aldrich) and transferred onto a nitrocellulose membrane (GE Healthcare Life Sciences, Little Chalfont, UK). .. The membrane was blocked with 5% skimmed milk and incubated with PBS containing Tween 20 and the following primary antibodies at 1:500 dilution: Monoclonal/polyclonal mouse/rabbit anti-human proliferating cell nuclear antigen (PCNA) (cat no. sc-25280), p21 (sc-397), p27 (sc-393380), p53 (sc-126), Bcl-2 (sc-7382), Bcl-2-associated X (Bax) (sc-7480), AIF (sc-13116), Cyt-c (sc-13561), cyclooxygenase (COX)4 (sc-292052), tubulin (sc-9104), histone (sc-10806), glucose‐regulated protein (GRP)94 (sc-11402), GRP78 (sc-1050), CCAAT-enhancer-binding protein homologous protein (CHOP) (sc-575) and GAPDH (sc-365062) (Santa Cruz Biotechnology, Dallas, TX, USA) and cyclin-dependend kinase (CDK)4 (cat no. 12790), cyclin D1 (1044), phosphorylated (p)-PERK (3179), PERK (3192), eIF2α (9722), p-eIF2α (3597), ATF4 (11815), IRE1α (3294), p-ASK (3765), ASK (37626S), p-p38 (4511), p38 (9213), ATF6 (11815S), p-PI3K (4288S), PI3K (4249S), p-AKT (4060P), AKT (#2920S), mammalian target of rapamycin (mTOR) (2983S) and p-mTOR (5536S) (Cell Signaling Technology, Inc., Danvers, MA, USA) overnight at 4 ̊C. .. After washing with Tris-buffered saline containing 1% Tween 20 (Sigma-Aldrich), membranes were incubated with the horseradish peroxidase-conjugated: Goat anti-rabbit immunoglobulin (Ig)G (cat. no. sc‐34661) or donkey anti-goat IgG (cat. no. sc-362265; Santa Cruz Biotechnology, Inc.) for 2 h at 4 ̊C.

    Incubation:

    Article Title: CXC195 induces apoptosis and endoplastic reticulum stress in human hepatocellular carcinoma cells by inhibiting the PI3K/Akt/mTOR signaling pathway.
    Article Snippet: Protein samples from the HepG2 cells extracts were separated using 8% SDS-PAGE (high molecular weight) or 10% SDS-PAGE (regular molecular weight) (Sigma-Aldrich) and transferred onto a nitrocellulose membrane (GE Healthcare Life Sciences, Little Chalfont, UK). .. The membrane was blocked with 5% skimmed milk and incubated with PBS containing Tween 20 and the following primary antibodies at 1:500 dilution: Monoclonal/polyclonal mouse/rabbit anti-human proliferating cell nuclear antigen (PCNA) (cat no. sc-25280), p21 (sc-397), p27 (sc-393380), p53 (sc-126), Bcl-2 (sc-7382), Bcl-2-associated X (Bax) (sc-7480), AIF (sc-13116), Cyt-c (sc-13561), cyclooxygenase (COX)4 (sc-292052), tubulin (sc-9104), histone (sc-10806), glucose‐regulated protein (GRP)94 (sc-11402), GRP78 (sc-1050), CCAAT-enhancer-binding protein homologous protein (CHOP) (sc-575) and GAPDH (sc-365062) (Santa Cruz Biotechnology, Dallas, TX, USA) and cyclin-dependend kinase (CDK)4 (cat no. 12790), cyclin D1 (1044), phosphorylated (p)-PERK (3179), PERK (3192), eIF2α (9722), p-eIF2α (3597), ATF4 (11815), IRE1α (3294), p-ASK (3765), ASK (37626S), p-p38 (4511), p38 (9213), ATF6 (11815S), p-PI3K (4288S), PI3K (4249S), p-AKT (4060P), AKT (#2920S), mammalian target of rapamycin (mTOR) (2983S) and p-mTOR (5536S) (Cell Signaling Technology, Inc., Danvers, MA, USA) overnight at 4 ̊C. .. After washing with Tris-buffered saline containing 1% Tween 20 (Sigma-Aldrich), membranes were incubated with the horseradish peroxidase-conjugated: Goat anti-rabbit immunoglobulin (Ig)G (cat. no. sc‐34661) or donkey anti-goat IgG (cat. no. sc-362265; Santa Cruz Biotechnology, Inc.) for 2 h at 4 ̊C.



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